It has been reported that human adipose tissue also contains a population of non‐characterized cells that are able to undergo adipogenic, osteogenic, chondrogenic or miogenic differentiation in vitro. 2000).Īdipose tissue is an active endocrine organ secreting, among other adipokines, various pro‐angiogenic substances, cytokines and growth factors (reviewed by Trayhurn & Beattie 2001). However, such cells may also participate in pathological processes associated with inflammation and/or carcinogenesis ( Halvorsen et al. It has been also demonstrated, that a certain number of undifferentiated cells residing in mature organs play an important role in tissue remodelling and repair during adolescence. Several recent publications have indicated that in the body, adipose tissue mass is dependent and regulated by neo‐angiogenesis ( Rupnick et al. Our work provides a clear model for analysing the differentiation capacity of SVF cells. Conclusions: Here, we show that differentiation of SVF cells to endothelial cells or adipocyte‐like cells depended on the medium used. SVF cells were found to work together with human umbilical vein endothelial cells to form capillary networks. Results: Differentiation of human SVF cells towards endothelium was stimulated by the presence of serum and absence of adipogenic factors, documented by the pattern of gene expression as well as different functional in vitro assays. A number of tests were performed to verify their differentiation. Cells were characterized by presence of surface antigens by flow cytometry, and by expression of genes characteristic for endothelial cells or for adipocytes, quantitative real‐time polymerase chain reaction. Materials and methods: Stromal vascular fraction cells were isolated according to modified Hauner's method and after adaptation they were cultured in pro‐angiogenic or pro‐adipogenic medium. The aim of this study was to determine conditions for promoting differentiation of human adipose tissue progenitors towards endothelial cells, as well as to show that SVF cells cooperate with differentiated endothelium in capillary network formation. It has been shown that human adipose tissue contains a population of non‐characterized cells, called stromal‐vascular fraction (SVF) cells, which are able to differentiate into several lineages.
Objectives: Angiogenesis, the process of formation of blood vessels, is essential for many physiological as well as pathological processes.
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